Phytochemical Screening and Antibacterial Activity of Dendrobium from Papua Against Eschericia coli and Staphylococcus aureus

Orchid found as one of medicinal plant in some areas, especially Dendrobium. Some species were investigated its bioactive compound, and antibacterial activity, but the information about activity Papuan Dendrobium is still limited. The aims this research to determine phytochemical constituents ethanolic extract fraction from Papua against Escherichia coli Staphylococcus aureus. There three namely: D. spectabile, violaceoflavens, antennatum used study. Dried simplisia was macerated using ethanol, then tested for content. Total fractionated with different solvents, ethyl acetate hexane. Disc diffusion assay examine activities total fractions. positive flavonoid, alkaloid tanin, except spectabile stem only tannin. No saponin plants. leaves showed highest S. aureus diameter inhibition zone 20.54 + 1.47 mm (strong category). Other had moderate both E.coli S aureus zone 6-9 mm. present work indicates that potential be developed drug design research.  Key words: activity; Papua; Dendrobium; E. coli; aureus.Â